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Angiogenesis Analyzer for ImageJ

Saturday 20 October 2012, by Gilles Carpentier

  Table of contents  

This tool was presented at the poster session ( abstract ) of the

ImageJ User and Developer Conference 2012


 Description:


The "Angiogenesis Analyzer" [1] allows analysis of cellular networks. Typically, it can detect and analyze the pseudo vascular organization of endothelial cells cultured in gel medium.

Among the tools used to evaluate the anti-angiogenesis properties of drugs, the most used is the in vitro differentiation of primary endothelial cells culture in gel (Endothelial Tube Formation Assay (ETFA)) [2]. In suitable culture conditions these cells form structures that can branch and mimic a pseudo capillary in vitro formation. At later stage this differentiation can lead to a meshed network from different mesh sizes. Although widely used, the interpretation of this assay still remains a problem, especially to obtain a quantitative evaluation of the vessels-like net organization. The Angiogenesis Analyzer is a simple tool to quantify the ETFA experiment images by extracting characteristic information of the network.

Above: phase contrast image of HUVEC network, analyzed by the Angiogenesis Analyzer for ImageJ. The same analysis can be obtained using a fluorescent staining by calcein.

 Collaborations - image and thematic providers:


- Ségolène Ferratge, Dr. Fabien Chevalier and Dr. Georges Uzan. INSERM U1197, Hôpital Paul Brousse, Villejuif. France. [3]
- Dr Cédric ALLIER. CEA-Leti - MINATEC Campus, Grenoble, France. [4]
- Geoffrey Estaban. IPRASENSE, Cap Alpha, Clapiers, France. www.iprasense.com [4].
- Dacha Gholobova, Lieselot Decroix and Prof. dr. ir. Lieven Thorrez. Dept. Development and Regeneration @Kulak, KU Leuven, Kortrijk. Belgium. [5]
- Dr. Arvind P. Pathak and Malvi Hemani, Depts. of Radiology and Oncology, The Johns Hopkins University School of Medicine, Baltimore. USA. Web: www.pathaklab.org. [6]


- Dr Sarah Berndt and Prof Muriel Cuendet. Pharmacognosie, Phytochimie et produits naturels bioactifs. Section des Sciences Pharmaceutiques. Université de Genève, Université de Lausanne Sciences II. Switzerland. [7] [8]
- Dr. Valeria Samarelli  and Marco Arese, Associate Professor of Biochemistry, Department of Oncological Sciences - University of Turin - School of Medecine - Institute for Cancer Research and Treatment (IRCC). Italy.  [9]

History:

- First version: 15 October 2012

Click the "Update and Version Infos" icon to look for new versions:

Footnotes

[1] Gilles Carpentier. Contribution: Angiogenesis Analyzer. ImageJ News, 5 October 2012.

[2] L. Montesano, R. Orci and P. Vassalli, “In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices”, J Cell Biol 97, pp. 1648–52, 1983.

[3] Chevalier F, Lavergne M, Negroni E, Ferratge S, Carpentier G, Gilbert-Sirieix M, Siñeriz F, Uzan G, Albanese P. Glycosaminoglycan mimetic improves enrichment and cell functions of human endothelial progenitor cell colonies. Stem Cell Res. May;12(3):703-15, 2014. Abstract.

[4] Allier C, Esteban G, Carpentier G, Van Der Sanden B, Vinjimore Kesavan S, Coutard J-G, Bordy T, Momey F, Herve L, Hamard L, Di Natale C, Wion D, Dinten J-M. Video Lensfree Microscopy for the Quantification of Endothelial Cellular Network Formation. 6ème Congrès de la Société Française d’Angiogenèse. Paris Collège de France – Septembre 24-25, 2015.

[5] Gholobova D, Decroix L, Van Muylder V, Desender L, Gerard M, Carpentier G, Vandenburgh H, Thorrez L. Endothelial Network Formation Within Human Tissue-Engineered Skeletal Muscle. Tissue Eng Part A. 2015 Sep 1. Abstract

[6] Fortenberry YM, Brandal SM, Carpentier G, Hemani M, Pathak AP. Intracellular Expression of PAI-1 Specific Aptamers Alters Breast Cancer Cell Migration, Invasion and Angiogenesis. PLoS One. 2016 Oct 18;11(10):e0164288. Abstract.

[7] Sakr OS, Berndt S, Carpentier G, Cuendet M, Jordan O, Borchard G. Arming embolic beads with anti-VEGF antibodies and controlling their release using LbL technology. J Control Release. 2016 Feb 28;224:199-207. Abstract.

[8] Issa ME, Berndt S, Carpentier G, Pezzuto JM, Cuendet M. Bruceantin inhibits multiple myeloma cancer stem cell proliferation. Cancer Biol There. 2016 Jul 19:0. Abstract.

[9] Samarelli AV, Riccitelli E, Bizzozero L, Nunes Silveira T, Seano G, Pergolizzi M, Vitagliano G, Cascone I, Carpentier G, Bottos A, Primo L, Bussolino F, Arese M. Neuroligin 1 induces blood vessels maturation by cooperating with the α6 integrin. J Biol Chem. May 23. pii: jbc.M113.530972. 2014. Abstract

logoij ImageJ (http://rsb.info.nih.gov/ij/) is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.1 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux. The author, Wayne Rasband (wayne@codon.nih.gov), is at the Research Services Branch, National Institute of Mental Health, Bethesda, Maryland, USA.
Gilles Carpentier, Faculte des Sciences et Technologie,
Universite Paris Est Creteil Val-de-Marne, France.

Special thanks to Alessandra Albano for the English correction of these sites.
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