Gilles Carpentier Research Web Site: Computer Image Analysis 
- Description, p1
- Collaborations - image (...), p1
- Installation, p2
- Documentation, p3
- Tutorial, p4
- Measurements, p5
- References, p6
This tool was presented at the poster session (abstract) of the
ImageJ User and Developer Conference 2012
Description:
Among the tools used to evaluate the anti-angiogenesis properties of drugs, the most used is the in vitro differentiation of primary endothelial cells culture in gel (Endothelial Tube Formation Assay (ETFA)) [2]. In suitable culture conditions these cells form structures that can branch and mimic a pseudo capillary in vitro formation. At later stage this differentiation can lead to a meshed network from different mesh sizes. Although widely used, the interpretation of this assay still remains a problem, especially to obtain a quantitative evaluation of the vessels-like net organization. The Angiogenesis Analyzer is a simple tool to quantify the ETFA experiment images by extracting characteristic information of the network.
Above: phase contrast image of HUVEC network, analyzed by the Angiogenesis Analyzer for ImageJ. The same analysis can be obtained using a fluorescent staining by calcein.
Collaborations - image and thematic providers:
Elisabeth VAGANAY et Forence RUGGIERO, Matrix Biology and Pathology, Institut de Génomique Fonctionnelle de Lyon, ENS de lyon, Université de Lyon, CNRS UMR 5242. [3].
Dr. Richard Cook and Pedro Bastos, Dental Institute, Guy’s Hospital, King’s College London, UK. [4].
Rachna Mirchandani,
Savini Thrikawala, Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, Houston, TX, USA, and Dr. Maria Bondesson Intelligent Systems Engineering, Indiana University, Bloomington, IN, USA.
Dr. Fabien Chevalier, Dr. Ségolène Ferratge and Dr. Georges Uzan. INSERM U1197, Hôpital Paul Brousse, Villejuif. France. [5] [6] [7].
Dr Cédric ALLIER. CEA-Leti - MINATEC Campus, Grenoble, France. [8].
Geoffrey Estaban. IPRASENSE, Cap Alpha, Clapiers, France. www.iprasense.com [9].
Dacha Gholobova, Lieselot Decroix and Prof. dr. ir. Lieven Thorrez. Dept. Development and Regeneration @Kulak, KU Leuven, Kortrijk. Belgium. [10] [11]
Dr. Arvind P. Pathak and Malvi Hemani, Depts. of Radiology and Oncology, The Johns Hopkins University School of Medicine, Baltimore. USA. Web: www.pathaklab.org. [12]
Dr Sarah Berndt and Prof Muriel Cuendet. Pharmacognosie, Phytochimie et produits naturels bioactifs. Section des Sciences Pharmaceutiques. Université de Genève, Université de Lausanne Sciences II. Switzerland. [13] [14] [15] [16] [17] [18].
Dr. Valeria Samarelli and Marco Arese, Associate Professor of Biochemistry, Department of Oncological Sciences - University of Turin - School of Medecine - Institute for Cancer Research and Treatment (IRCC). Italy. [19]
History:
First version: 15 October 2012
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