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Wednesday 21 October 2015, by Gilles Carpentier

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References:

- LFM-init-rec: Lensfree raw acquisition of human umbilical vein endothelial cells cultured on Matrigel. The field of view is 29.4 mm2

- LFM-treated-rec: Holographic reconstruction of the recorded image shown in (a). The results is a modulus image which depicts the cells in dark gray over a light grey background.

The holographic reconstruction consists first in back-propagating the light intensity recorded by the sensor through the Fresnel function. The recovered complex image is however strongly affected by the so-called ‘twin image’, an artefact that results from a lack of phase information in the acquisition process. To retrieve the phase in the sensor plane and consequently diminish the ‘twin-image’ artefact in the reconstructed images, we used a phase-retrieval algorithm. The purpose of such an algorithm is to recover the complex image of the sample from the phase-less recorded holographic image. It allows to refine the estimate of the complex image in the sensor plane by iteratively applying update rules in a gradient descent scheme.

Credits : Cell culture: F.Navarro (ab), M. Menneteau (ab), Holographic reconstruction: L. Hervé (ab), C. Allier (ab), a Univ. Grenoble Alpes, F‐38000 Grenoble, France. b CEA LETI MlNATEC Campus, F-38054 Grenoble, France.

- LFM-analyzed-rec: Image " LFM-treated-rec" analyzed by a customized version of the Angiogenesis Analyzer.

- microcosme2: Fluorescent algae and other microorganisms. Olympus IX80 microscope coupled to an ORCA R2 Hamamatsu CCD camera. Sample source; Iglika Christova, image acquisition and processing, Gilles Carpentier.

- lens-free-init2 (HUVEC Lensfree Microscopy), and lens-free-obj2 (HUVEC Lensfree Microscopy + Analysis). HUVEC network acquired by lens free microscopy and analyzed by a cutomized version of the Angiogenesis Analyzer. Details and timelapse here.

- NiceCells2 (Fluorescent Cell - Tubulin labeling (green)) Image source : slide through the courtesy of Angelica Keller, image acquisition from the author. BH2 Olympus microscope and Scion CCD CFW-1310M Camera. Project link.

- Huvec Phase Contrast, Huvec Phase Contrast + Analysis, Huvec Fluo, Huvec Fluo + Analysis, are huvec networks analyzed with the Angiogenesis Analyzer.

- HistoMuscle-4Channels and HistoMuscle-3Channels : Muscle slice; immunolabeling from Elisa Negroni, image acquisition from Emilie Henault. IX81 inverted Olympus microscope and Orca Hamamatsu R2 CCD camera. Project link.

- Myoblast-init-2, Myoblast-init-Deblured-2, Myoblast-init-Deblured-fft-fft-2 : Different improvements of a laser scanning transmission image. Image from the author. Project link.

- Nerve image from the courtesy of Dr  Brigitte Blondet. Device for the image acquisition: monochrome CMOS Sound Vision camera mounted onto an Olympus BH-2 microscope. Project link

- Rat myotube culture immunostaining. The example contains a triple labeling of a differentiated mouse myogenic cell line. Cell culture and immunochemistry; Juliette Peltzer, images from the courtesy of Dr Angelica Keller. Project links, here and here.

- « Array » and « Analyzed Array » are protein arrays analyzed by the Protein Array Analyzer, ImageJ conference.

logoij ImageJ (http://rsb.info.nih.gov/ij/) is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.1 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux. The author, Wayne Rasband (wayne@codon.nih.gov), is at the Research Services Branch, National Institute of Mental Health, Bethesda, Maryland, USA.
Gilles Carpentier, Faculte des Sciences et Technologie,
Universite Paris Est Creteil Val-de-Marne, France.

Special thanks to Alessandra Albano for the English correction of these sites.
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