Gilles Carpentier Research Web Site: Computer Image Analysis
Image analysis tools in biology and biochemistry using ImageJ

Home > Protein Array Analyzer for ImageJ

Protein Array Analyzer for ImageJ

Friday 15 October 2010, by Gilles Carpentier

  Table of contents  

 Video Tutorials:

- Manually dot by dot analysis of a demo dot blot

- Demo dot blot analysis as a grid of dots

- Demo set of protein array analysis

  • Step 1 - group the set of array images to analyse into a same folder, and analyse each of them as follows.

  • Step 2 - once every analysis performed, masterize and (or) set internal references as follows. Up to 4 control and internal reference dots can be averaged and used for the master normalization.

See the Tutorial section for more details

logoij ImageJ ( is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.1 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux. The author, Wayne Rasband (, is at the Research Services Branch, National Institute of Mental Health, Bethesda, Maryland, USA.
Gilles Carpentier, Faculte des Sciences et Technologie,
Universite Paris Est Creteil Val-de-Marne, France.

Special thanks to Alessandra Albano for the English correction of these sites.
Computer Data Acquisition
for Biochemistry Practice Works
Image analysis tools in biology
and biochemistry using ImageJ