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Image analysis tools in biology and biochemistry using ImageJ

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Image analysis and processing tool developments using the ImageJ software:

Thursday 2 April 2009, by Gilles Carpentier

  Table of contents  

 Graphical interface:

The « Image, Stack and Timelapse Arrow Labelling toolset » [5] has been developped both to simplify user’s work, and to make the first demonstration of graphical interface in macro language: a preview window shows user’s choices, before applying them to an image. The aim of the tool is to annotate images or time-lapse movies with customisable arrows (Gray C et al [6]). The two examples below show the advantages of this method.

Above: standard pop-up menu, used to set the user’s choices of an arrow maker tool [7].

The next picture shows the advantages of an intuitive graphical interface, to set the arrow direction instead of selecting the cardinal names. In the same way, color and size can be immediately seen at the target point into a preview sample of the working image.

Footnotes

[5Carpentier G. Contribution: the Image, Stack and Timelapse Arrow Labelling toolset (online documentation), which allows the user to draw arrows on images and stacks. ImageJ News, May 2007.

[6Gray C, Packham IM, Wurmser F, Eastley NC, Hellewell PG, Ingham PW, Crossman DC, Chico TJ. Ischemia is not required for arteriogenesis in zebrafish embryos. Arterioscler Thromb Vasc Biol. 2007 Oct; 27(10): 2135-4.

[7Carpentier G. Contribution: the Arrow Maker Tool macro. Examples are at image.bio.methods.free.fr/Arrowsdoc.html. ImageJ News, June 2005.

logoij ImageJ (http://rsb.info.nih.gov/ij/) is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.1 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux. The author, Wayne Rasband (wayne@codon.nih.gov), is at the Research Services Branch, National Institute of Mental Health, Bethesda, Maryland, USA.
Gilles Carpentier, Faculte des Sciences et Technologie,
Universite Paris Est Creteil Val-de-Marne, France.

Special thanks to Alessandra Albano for the English correction of these sites.
Computer Data Acquisition
for Biochemistry Practice Works

Image.Bio.Methods@free.fr
Image analysis tools in biology
and biochemistry using ImageJ