|Gilles Carpentier Research Web Site: Computer Image Analysis - Methods and Examples.|
|--------||--Dot Blot Analyzer for ImageJ § - Documentation:--------|
- Find a pertinant circle radius by clicking onto the circle containing radius icone. ---------
- Adjust the cursor circle size using the "+" and "-” sub-buttons.
|- Normalize the histogram from the "stretch curve" icon. |
- Adjust the saturation level using the "+" and "-” sub-buttons.
|- Choose the appropriate Look Up Table (LUT) by clicking into the LUT ramps icone.--|
|- Restore the initial preview properties with the counterclockwise symbol.|
|- Click "Add" to generate a tab with the current value given by the cursor, "Remove" to delete it |
(CTRL click undo the delete action).
- Erase all the measurements of the tab by the red "X". This action in undo by "CTRL click".---
|- Adjust the column ("nCol.") and the line ("nLin.") numbers, corresponding to the total or sub-area |
dot blot to analyse.
|- Set the three required dots forming angle (hight-left, hight-right and low-left) using the three |
green cross hair icones.
|- Marks can be adjusted, by
re-selecting the appropriate cross hair symbol (Green cross hair: mark |
to set. Red cross hair: set mark. Orange cross hair: mark in setting).
|- Get a modelled image of the grid analysis by clicking on "Model".|
|- "Shift" between the grid measurements mode and the single measurements mode can be performed |
by clicking onto "Measurements:" and "Grid:".
|- "Cancel" box closes every thing concerning one analysis, including the Palette.|
|- "Erase" box removes every drawn dot contours. They can be redrawn by clicking onto "Measurements:" |
|- "End: Quit": finish an analysis (Deactivate the Palette, keeping an image or the current drawings).|
§ ImageJ (http://rsb.info.nih.gov/ij/) is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.1 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux. The author, Wayne Rasband (firstname.lastname@example.org), is at the Research Services Branch, National Institute of Mental Health, Bethesda, Maryland, USA.
Gilles Carpentier, Faculte des Sciences et Technologies,
Universite Paris 12 Val-de-Marne, France.
and ImageJ contributions
|Computer Data Acquisition
for Biochemistry Practice Works