Carpentier's Web Site:
Data Acquisition for Biochemistry Practice Works
practical, quick, step by step illustrated procedure to "build" a
Clark electrode :
This page describes a
step by step procedure, allowing to make an operational Clark probe
in a few minutes. The device used comes from hansatech-instruments.
More details about
the physico-chemical principle are avaible on the "Experimental
membrane" sensor :
the membrane :
First, put a
drop of KCl onto the cathode, and put drops of KCl onto the annular
anode to make an uniform film of KCl solution (see arrows on the
Put a piece
of cigaret paper (prefer thin paper, don't take the border containing
glue) on the top of the electode, cover by a piece of polypropylene,
and gently place the O-ring joint, avoiding the formation of bubbles
below the membranes.
applicator onto the O-ring and "seale" the membranes. The membranes
have to be free of air bubbles.
Aspect of the
electrode ready to be assemble to the chamber:
following pictures show the assembly of the electrode and the
The electrode ready to use, on a magnetic stirrer, connected to the
control device and to a thermostated circulation of water
Return to the
Special thanks to Alessandra Albano for her participation to
the English correction of this page.
author: Gilles Carpentier. You can mail me if you are interested in
Carpentier, Faculté des Sciences et Technologies,
Université Paris 12 Val-de-Marne, France.
Carpentier's Web Site: "Computer
Data Acquisition for Biochemistry Practice Works: Methods and