Gilles
Carpentier's Web Site:
Computer
Data Acquisition for Biochemistry Practice Works
Methods
and Examples
A
practical, quick, step by step illustrated procedure to "build" a
Clark electrode :
This page describes a
step by step procedure, allowing to make an operational Clark probe
in a few minutes. The device used comes from hansatech-instruments.
More details about
the physico-chemical principle are avaible on the "Experimental
Biosciences"
web site.
The "free
membrane" sensor :
Montage of
the membrane :
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First, put a
drop of KCl onto the cathode, and put drops of KCl onto the annular
anode to make an uniform film of KCl solution (see arrows on the
picture above)
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-------
Put a piece
of cigaret paper (prefer thin paper, don't take the border containing
glue) on the top of the electode, cover by a piece of polypropylene,
and gently place the O-ring joint, avoiding the formation of bubbles
below the membranes.
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-------
Apply the
applicator onto the O-ring and "seale" the membranes. The membranes
have to be free of air bubbles.
Aspect of the
electrode ready to be assemble to the chamber:
The three
following pictures show the assembly of the electrode and the
chamber:
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-------
The electrode ready to use, on a magnetic stirrer, connected to the
control device and to a thermostated circulation of water
(arrows)
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Return to the
Clak
electrode
main page
Special thanks to Alessandra Albano for her participation to
the English correction of this page.
Page
author: Gilles Carpentier. You can mail me if you are interested in
more details:
Address:
Gilles
Carpentier, Faculté des Sciences et Technologies,
Université Paris 12 Val-de-Marne, France.
Gilles
Carpentier's Web Site: "Computer
Data Acquisition for Biochemistry Practice Works: Methods and
Examples"
