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Computer Data Acquisition for Biochemistry Practice Works
Methods and Examples
A practical, quick, step by step illustrated procedure to "build" a Clark electrode :

This page describes a step by step procedure, allowing to make an operational Clark probe in a few minutes. The device used comes from hansatech-instruments.

More details about the physico-chemical principle are avaible on the "Experimental Biosciences" web site.

The "free membrane" sensor :

Montage of the membrane :


First, put a drop of KCl onto the cathode, and put drops of KCl onto the annular anode to make an uniform film of KCl solution (see arrows on the picture above)


Put a piece of cigaret paper (prefer thin paper, don't take the border containing glue) on the top of the electode, cover by a piece of polypropylene, and gently place the O-ring joint, avoiding the formation of bubbles below the membranes.

Apply the applicator onto the O-ring and "seale" the membranes. The membranes have to be free of air bubbles.
Aspect of the electrode ready to be assemble to the chamber:

The three following pictures show the assembly of the electrode and the chamber:


The electrode ready to use, on a magnetic stirrer, connected to the control device and to a thermostated circulation of water (arrows)

Return to the Clak electrode main page

Special thanks to Alessandra Albano for her participation to the English correction of this page.

Page author: Gilles Carpentier. You can mail me if you are interested in more details:

Address: Gilles Carpentier, Faculté des Sciences et Technologies, Université Paris 12 Val-de-Marne, France.

Gilles Carpentier's Web Site: "Computer Data Acquisition for Biochemistry Practice Works: Methods and Examples"

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