Image analysis tools in biology and biochemistry using ImageJ
Angiogenesis Analyzer for ImageJ
Saturday 20 October 2012, by
This tool was presented at the poster session ( abstract
) of the
The "Angiogenesis Analyzer"  allows analysis of cellular networks. Typically, it can detect and analyze the pseudo vascular organization of endothelial cells cultured in gel medium
Among the tools used to evaluate the anti-angiogenesis properties of drugs, the most used is the in vitro differentiation of primary endothelial cells culture in gel (Endothelial Tube Formation Assay (ETFA)) . In suitable culture conditions these cells form structures that can branch and mimic a pseudo capillary in vitro formation. At later stage this differentiation can lead to a meshed network from different mesh sizes. Although widely used, the interpretation of this assay still remains a problem, especially to obtain a quantitative evaluation of the vessels-like net organization. The Angiogenesis Analyzer is a simple tool to quantify the ETFA experiment images by extracting characteristic information of the network.
Above: phase contrast image of HUVEC network, analyzed by the Angiogenesis Analyzer for ImageJ. The same analysis can be obtained using a fluorescent staining by calcein.
First version: 15 October 2012
Click the "Update and Version Infos" icon to look for new versions:
 Gilles Carpentier. Contribution: Angiogenesis Analyzer. ImageJ News, 5 October 2012.
 L. Montesano, R. Orci and P. Vassalli, “In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices”, J Cell Biol 97, pp. 1648–52, 1983.
|| ImageJ (http://rsb.info.nih.gov/ij/) is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.1 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux. The author, Wayne Rasband (firstname.lastname@example.org), is at the Research Services Branch, National Institute of Mental Health, Bethesda, Maryland, USA.