Gilles Carpentier Research Web Site: Computer Image Analysis
Image analysis tools in biology and biochemistry using ImageJ

Home page > Angiogenesis Analyzer for ImageJ

Angiogenesis Analyzer for ImageJ

Saturday 20 October 2012, by Gilles Carpentier

  Table of contents  

This tool was presented at the poster session ( abstract ) of the

ImageJ User and Developer Conference 2012


 Description:


The "Angiogenesis Analyzer" [1] allows analysis of cellular networks. Typically, it can detect and analyze the pseudo vascular organization of endothelial cells cultured in gel medium.

Among the tools used to evaluate the anti-angiogenesis properties of drugs, the most used is the in vitro differentiation of primary endothelial cells culture in gel (Endothelial Tube Formation Assay (ETFA)) [2]. In suitable culture conditions these cells form structures that can branch and mimic a pseudo capillary in vitro formation. At later stage this differentiation can lead to a meshed network from different mesh sizes. Although widely used, the interpretation of this assay still remains a problem, especially to obtain a quantitative evaluation of the vessels-like net organization. The Angiogenesis Analyzer is a simple tool to quantify the ETFA experiment images by extracting characteristic information of the network.

Above: phase contrast image of HUVEC network, analyzed by the Angiogenesis Analyzer for ImageJ. The same analysis can be obtained using a fluorescent staining by calcein.


 Collaborations - image and thematic providers:


- Dr. Fabien Chevalier and Dr. Georges Uzan. INSERM U972, Hôpital Paul Brousse, Villejuif. France.
- Dr. José Courty and Dr. Ilaria Cascone. Laboratoire CRRET EAC CNRS 7149, Faculté des Sciences et Technologie, Université Paris Est Créteil. France.
- Lieselot Decroix and Prof. dr. ir. Lieven Thorrez. Dept. Development and Regeneration @Kulak, KU Leuven, Kortrijk. Belgium.
- Dr. Arvind P. Pathak and Malvi Hemani, Depts. of Radiology and Oncology, The Johns Hopkins University School of Medicine, Baltimore. USA. Web: www.pathaklab.org
- Dr. Valeria Samarelli  and Marco Arese, Associate Professor of Biochemistry, Department of Oncological Sciences - University of Turin - School of Medecine - Institute for Cancer Research and Treatment (IRCC). Italy.
- Dr. Sarah Berndt and Prof. Muriel Cuendet. Pharmacognosie, Phytochimie et produits naturels bioactifs. Section des Sciences Pharmaceutiques. Université de Genève, Université de Lausanne Sciences II. Switzerland.


History:

- First version: 15 October 2012

Click the "Update and Version Infos" icon to look for new versions:

Footnotes

[1] Gilles Carpentier. Contribution: Angiogenesis Analyzer. ImageJ News, 5 October 2012.

[2] L. Montesano, R. Orci and P. Vassalli, “In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices”, J Cell Biol 97, pp. 1648–52, 1983.

logoij ImageJ (http://rsb.info.nih.gov/ij/) is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.1 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux. The author, Wayne Rasband (wayne@codon.nih.gov), is at the Research Services Branch, National Institute of Mental Health, Bethesda, Maryland, USA.
Gilles Carpentier, Faculte des Sciences et Technologie,
Universite Paris Est Creteil Val-de-Marne, France.

Special thanks to Alessandra Albano for the English correction of these sites.
Computer Data Acquisition
for Biochemistry Practice Works

Image.Bio.Methods@free.fr
Image analysis tools in biology
and biochemistry using ImageJ
| Site Map |