This tool was presented at the poster session ( abstract ) of the
Among the tools used to evaluate the anti-angiogenesis properties of drugs, the most used is the in vitro differentiation of primary endothelial cells culture in gel (Endothelial Tube Formation Assay (ETFA)) . In suitable culture conditions these cells form structures that can branch and mimic a pseudo capillary in vitro formation. At later stage this differentiation can lead to a meshed network from different mesh sizes. Although widely used, the interpretation of this assay still remains a problem, especially to obtain a quantitative evaluation of the vessels-like net organization. The Angiogenesis Analyzer is a simple tool to quantify the ETFA experiment images by extracting characteristic information of the network.
Above: phase contrast image of HUVEC network, analyzed by the Angiogenesis Analyzer for ImageJ. The same analysis can be obtained using a fluorescent staining by calcein.
Dr. Fabien Chevalier and Dr. Georges Uzan. INSERM U972, Hôpital Paul Brousse, Villejuif. France.
Dr. José Courty and Dr. Ilaria Cascone. Laboratoire CRRET EAC CNRS 7149, Faculté des Sciences et Technologie, Université Paris Est Créteil. France.
Lieselot Decroix and Prof. dr. ir. Lieven Thorrez. Dept. Development and Regeneration @Kulak, KU Leuven, Kortrijk. Belgium.
Dr. Arvind P. Pathak and Malvi Hemani, Depts. of Radiology and Oncology, The Johns Hopkins University School of Medicine, Baltimore. USA. Web: www.pathaklab.org
Dr. Valeria Samarelli and Marco Arese, Associate Professor of Biochemistry, Department of Oncological Sciences - University of Turin - School of Medecine - Institute for Cancer Research and Treatment (IRCC). Italy.
Dr. Sarah Berndt and Prof. Muriel Cuendet. Pharmacognosie, Phytochimie et produits naturels bioactifs. Section des Sciences Pharmaceutiques. Université de Genève, Université de Lausanne Sciences II. Switzerland.
First version: 15 October 2012
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