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Angiogenesis Analyzer for ImageJ

Saturday 20 October 2012, by Gilles Carpentier

  Table of contents  

This tool was presented at the poster session ( abstract ) of the

ImageJ User and Developer Conference 2012


The "Angiogenesis Analyzer" [1] allows analysis of cellular networks. Typically, it can detect and analyze the pseudo vascular organization of endothelial cells cultured in gel medium.

Among the tools used to evaluate the anti-angiogenesis properties of drugs, the most used is the in vitro differentiation of primary endothelial cells culture in gel (Endothelial Tube Formation Assay (ETFA)) [2]. In suitable culture conditions these cells form structures that can branch and mimic a pseudo capillary in vitro formation. At later stage this differentiation can lead to a meshed network from different mesh sizes. Although widely used, the interpretation of this assay still remains a problem, especially to obtain a quantitative evaluation of the vessels-like net organization. The Angiogenesis Analyzer is a simple tool to quantify the ETFA experiment images by extracting characteristic information of the network.

Above: phase contrast image of HUVEC network, analyzed by the Angiogenesis Analyzer for ImageJ. The same analysis can be obtained using a fluorescent staining by calcein.

 Collaborations - image and thematic providers:

- Dr. Fabien Chevalier and Dr. Georges Uzan. INSERM U1197, Hôpital Paul Brousse, Villejuif. France.
- Dr. José Courty and Dr. Ilaria Cascone. Laboratoire CRRET EAC CNRS 7149, Faculté des Sciences et Technologie, Université Paris Est Créteil. France.
- Lieselot Decroix and Prof. dr. ir. Lieven Thorrez. Dept. Development and Regeneration @Kulak, KU Leuven, Kortrijk. Belgium.
- Dr. Arvind P. Pathak and Malvi Hemani, Depts. of Radiology and Oncology, The Johns Hopkins University School of Medicine, Baltimore. USA. Web:
- Dr. Valeria Samarelli  and Marco Arese, Associate Professor of Biochemistry, Department of Oncological Sciences - University of Turin - School of Medecine - Institute for Cancer Research and Treatment (IRCC). Italy.
- Dr. Sarah Berndt and Prof. Muriel Cuendet. Pharmacognosie, Phytochimie et produits naturels bioactifs. Section des Sciences Pharmaceutiques. Université de Genève, Université de Lausanne Sciences II. Switzerland.


- First version: 15 October 2012

Click the "Update and Version Infos" icon to look for new versions:


[1] Gilles Carpentier. Contribution: Angiogenesis Analyzer. ImageJ News, 5 October 2012.

[2] L. Montesano, R. Orci and P. Vassalli, “In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices”, J Cell Biol 97, pp. 1648–52, 1983.

logoij ImageJ ( is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.1 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux. The author, Wayne Rasband (, is at the Research Services Branch, National Institute of Mental Health, Bethesda, Maryland, USA.
Gilles Carpentier, Faculte des Sciences et Technologie,
Universite Paris Est Creteil Val-de-Marne, France.

Special thanks to Alessandra Albano for the English correction of these sites.
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